Bioinformatics Solutions for CRISPR

Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) sequences are specialised sections of DNA, playing a key role in antiviral defences.

Fios are well-placed to help you get the most out of your CRISPR-generated data, as our approaches allow you to go deeper into the analysis of data produced. We can help at any stage of your study, from the design through to the analysis of on- and off-target effects.

We have worked on a range of related projects including:

• Multivariate analysis across RNA-Seq and SNP/CNV data, alongside efficacy data from over 800 CRISPR screens.
• Identification of sensitivities to CRISPR knockouts in cell lines of interest.

CRISPR Services

Fios can help with a range of CRISPR-generated data such as:

• Gene expression and GWAS
• Epigenetics
• Microbiome
• Metabolomics and proteomics

We can analyse data taken from publicly available data sources in addition to datasets provided by clients.

Getting Your Data Ready For Analysis

We can analyse:

• Recent data, generated within your own laboratory or that of a 3rd party provider
• In-house historic data
• Data sourced from the public domain.

While we prefer to receive data in the raw format (eg .CEL files) we can also receive pre-normalised files.

If you have yet to generate the data, we can help by arranging for the generation of your data through one of our preferred laboratory service partners. Alternatively, we are able to source and mine data from available databases. We are happy to work with you at every stage of your study to ensure the best outcome for your research.

Our Reports

Our analysis reports at Fios are provided as an HTML link hosted on our secure server. The secure link leads the user to a password-protected HTML document which is clickable, searchable and dynamic. This format allows you to easily interrogate and explore your data. Additionally, all methods, tools and thresholds are fully documented within the report.